As far as the OP is concerned, no. I've played since the 80s & always managed to walk into bands I've wanted to join. I've never failed an audition & I've done a fair few sessions, hundreds of gigs and had a blast doing it all.
However, all that said I wish I'd learnt to read when I was younger. You have an extra string to your bow if you can & it will increase your options in terms of going for some sessions / gigs. It doesn't have to be a major barrier but it can only be of benefit if you have an extra skill.